Human BTLA (Q7Z6A9, 1 a.a. - 150 a.a.) partial recombinant protein with 6x His, Avi tag at C-terminal expressed in Expi293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (Expi293) expression system
Purification:
Ni-sepharose purification
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 0.1 EU/ug of the protein by LAL method.
Activity:
Immobilized BTLA (Human) Recombinant Protein (Cat # P6790) at 0.5 ug/mL (100 uL/well), dose response curve for Human HVEM, Fc Tag with the ED50 of 0.1 ug/mL determined by ELISA.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
BTLA (Human) Recombinant Protein (Cat # P6790) was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-45 kDa.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.
BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM