Human BTLA (NP_001078826.1, 31 a.a. - 134 a.a.) partial recombinant protein with Fc, 6x His tag at C-terminal expressed in HEK293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Ni-sepharose purification
Purity:
> 90% by SDS-PAGE
Endotoxin Level:
< 0.1 EU/ug of the protein by LAL method.
Activity:
Measured by its binding ability in a functional ELISA. Immobilized BTLA (Human) Recombinant Protein (Cat # P6789) at 3 ug/mL (100 uL/well) can bind Biotinylated Recombinant human HVEM with a linear range of 18-72 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
BTLA (Human) Recombinant Protein (Cat # P6789) was determined by SDS-PAGE with Coomassie Blue, showing a band at 50-60kDa.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.
BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM