Human CD276 (NP_079516, 29 a.a. - 245 a.a.) partial recombinant protein with 6x His tag at C-terminal expressed in HEK293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Ni-sepharose purification
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 0.1 EU/ug of the protein by LAL method.
Activity:
Measured by its binding ability in a functional ELISA. Immobilized CD276 (Human) Recombinant Protein (Cat # P6785) at 2 ug/mL (100 uL/well) can bind Anti-Human B7-H3 Antibody with a linear range of 1-4 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
CD276 (Human) Recombinant Protein (Cat # P6785) was determined by SDS-PAGE with Coomassie Blue, showing a band at 38-48 kDa.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM