CD276 (Human) Recombinant Protein
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Specification
Product Description
Human CD276 (NP_079516, 29 a.a. - 245 a.a.) partial recombinant protein with 6x His tag at C-terminal expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug of the protein by LAL method.
Activity
Measured by the binding ability in a functional ELISA. Immobilized CD276 (Human) Recombinant Protein (Cat # P6785) at 2 ug/mL (100 uL/well) can bind Anti-Human B7-H3 Antibody with a linear range of 1-4 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
CD276 (Human) Recombinant Protein (Cat # P6785) was determined by SDS-PAGE with Coomassie Blue, showing a band at 38-48 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — CD276
Entrez GeneID
80381Protein Accession#
NP_079516Gene Name
CD276
Gene Alias
B7-H3, B7H3
Gene Description
CD276 molecule
Omim ID
605715Gene Ontology
HyperlinkGene Summary
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM
Other Designations
B7 homolog 3|CD276 antigen
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