PDCD1LG2 (Human) Recombinant Protein
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Specification
Product Description
Human PDCD1LG2 (NP_079515.2, 20 a.a. - 219 a.a.) partial recombinant protein with 6x His tag at C-terminal expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug of the protein by LAL method.
Activity
Measured by the binding ability in a functional ELISA. Immobilized PDCD1LG2 (Human) Recombinant Protein (Cat # P6783) at 5 ug/mL (100 uL/well) can bind Recombinant Human PD-1 with a linear range of 0.3-1.2 ug/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
PDCD1LG2 (Human) Recombinant Protein (Cat # P6783) was determined by SDS-PAGE with Coomassie Blue, showing a band at 29-47 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — PDCD1LG2
Entrez GeneID
80380Protein Accession#
NP_079515.2Gene Name
PDCD1LG2
Gene Alias
B7DC, Btdc, CD273, MGC142238, MGC142240, PD-L2, PDCD1L2, PDL2, bA574F11.2
Gene Description
programmed cell death 1 ligand 2
Omim ID
605723Gene Ontology
HyperlinkOther Designations
OTTHUMP00000021030|PD-1-ligand 2|programmed death ligand 2
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