Human ICOS (Q9Y6W8, 21 a.a. - 141 a.a.) partial recombinant protein with hFc tag at C-terminal expressed in Expi293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (Expi293) expression system
Purification:
Protein A purification
Purity:
> 95% by Tris-Bis PAGE
Endotoxin Level:
< 1.0 EU/ug of the protein by LAL method.
Activity:
Immobilized Human B7-H2, His (BH7-HM472) at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for ICOS (Human) Recombinant Protein (Cat # P6778), hFc with the ED50 of 45.6 ng/mL determined by ELISA.
Quality Control Testing:
Tris-Bis PAGE with Coomassie Blue
ICOS (Human) Recombinant Protein (Cat # P6778) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage:
Tris-Bis PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq