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Last updated: 2022/5/15

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CD274 (Human) Recombinant Protein BioActive

  • Catalog # : P6776
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  • Specification
  • Product Description:
  • Human CD274 (Q9NZQ7, 19 a.a. - 238 a.a.) partial recombinant protein with 6x His tag at C-terminal expressed in Expi293 cells.
  • Host:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (Expi293) expression system
  • Purification:
  • Ni-sepharose purification
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • < 0.1 EU/ug of the protein by LAL method.
  • Activity:
  • Immobilized CD274 (Human) Recombinant Protein (Cat # P6776) at 5 ug/mL (100 uL/well), dose response curve for Anti PD-L1 Ab with the ED50 of 8.2 ng/mL determined by ELISA.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6776
    CD274 (Human) Recombinant Protein (Cat # P6776) was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-42 kDa.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4.
  • Storage Instruction:
  • Store the lyophilized protein at -20°C to -80°C for long term.
    After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • CD274
  • Gene Alias:
  • B7-H,B7H1,MGC142294,MGC142296,PD-L1,PDCD1L1,PDCD1LG1,PDL1
  • Gene Description:
  • CD274 molecule
  • Other Designations:
  • CD274 antigen,OTTHUMP00000021029,programmed cell death 1 ligand 1
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