Products

Product Description

Human MUC-1 (NP_001018016.1, 1 a.a. - 167 a.a.) partial recombinant protein with Fc and His tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Host

Human

Theoretical MW (kDa)

60-75

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purification

Ni-sepharose purification

Purity

> 97% by SDS-PAGE

Endotoxin Level

< 0.1 EU/ug

Activity

Measured by the ability to increase beta-catenin levels in the cytoplasm and nucleus of HCT116 human colon adenocarcinoma cells. 0.01-1 ng/mL of Recombinant Human MUC-1 can effectively increase beta-catenin levels.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

MUC-1 (Human) Recombinant Protein (Cat #P6772) was determined by SDS-PAGE with Coomassie Blue, showing a band at 60-75 kDa.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from PBS, pH 7.4.

Storage Instruction

Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.

Note

Result of activity analysis