LAG3 (Human) Recombinant Protein
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Specification
Product Description
Human LAG3 (P18627, 23 a.a. - 450 a.a.) partial recombinant protein with Fc tag at C-terminus expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
75-80
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Protein A purification
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
Immobilized Human FGL1 at 2 ug/mL (100 uL/well), dose response curve for Human LAG3,Fc with the EC50 of 21 ng/mL determined by ELISA.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
LAG3 (Human) Recombinant Protein (Cat #P6770) was determined by SDS-PAGE with Coomassie Blue, showing a band at 75-80 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — LAG3
Entrez GeneID
3902Protein Accession#
P18627Gene Name
LAG3
Gene Alias
CD223
Gene Description
lymphocyte-activation gene 3
Omim ID
153337Gene Ontology
HyperlinkGene Summary
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq
Other Designations
lymphocyte-activation protein 3
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