FCGR2A(Human) Recombinant Protein
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Specification
Product Description
Human FCGR2A (NP_001129691.1, 36 a.a. - 218 a.a.) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
32-38
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
Measured by the binding ability in a functional ELISA. Immobilized recombinant human CD32a at 1 ug/mL (100 uL/well) can bind biotinylated human IgG1 with a linear range of 30-250 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
FCGR2A(Human) Recombinant Protein (Cat #P6765) was determined by SDS-PAGE with Coomassie Blue, showing a band at 32-38 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — FCGR2A
Entrez GeneID
2212Protein Accession#
P12318Gene Name
FCGR2A
Gene Alias
CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, FcGR, IGFR2, MGC23887, MGC30032
Gene Description
Fc fragment of IgG, low affinity IIa, receptor (CD32)
Omim ID
146790Gene Ontology
HyperlinkGene Summary
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
Fc fragment of IgG, low affinity IIa, receptor|Fc fragment of IgG, low affinity IIa, receptor for (CD32)|Immunoglobulin G Fc receptor II|OTTHUMP00000032377
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