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Last updated: 2022/5/22

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CD47 (Human) Recombinant Protein BioActive

  • Catalog # : P6757
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  • Specification
  • Product Description:
  • Human CD47 recombinant protein with Fc and His tag at C-terminus expressed in HEK293 cells.
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 60
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purification:
  • Ni-sepharose purification
  • Purity:
  • > 95% by SDS-PAGE
  • Activity:
  • ELISA plate pre-coated by 2 ug/mL (100 uL/well) Human CD47, mFc-His tagged protein can bind its native ligand Human SIRP alpha, hFc-His tagged protein in a linear range of 3.3-26.37 ng/mL. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Human CD47, mFc-His tagged protein can bind Anti-CD47 in a linear range of 0.061-1.606 ng/mL.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6757
    CD47 (Human) Recombinant Protein (Cat #P6757) was determined by SDS-PAGE with Coomassie Blue, showing bands at 60 kDa
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4.
  • Storage Instruction:
  • Store the lyophilized protein at -20°C to -80°C for long term.
    After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 961
  • Gene Name:
  • CD47
  • Gene Alias:
  • IAP,MER6,OA3
  • Gene Description:
  • CD47 molecule
  • Gene Summary:
  • This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CD47 antigen,CD47 antigen (Rh-related antigen, integrin-associated signal transducer),CD47 glycoprotein,Rh-related antigen,antigen identified by monoclonal antibody 1D8,antigenic surface determinant protein OA3,integrin associated protein,integrin-associa
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