Products

Product Description

Human CD47 (Q08722, 19 a.a. - 139 a.a.) partial recombinant protein with His tag at C-terminus expressed in mammals.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Host

Human

Theoretical MW (kDa)

45-60

Form

Lyophilized

Preparation Method

Mammalian cell (Expi293) expression system

Purification

Ni-sepharose purification

Purity

> 95% by SDS-PAGE

Endotoxin Level

< 0.1 EU/ug

Activity

Immobilized Human CD47, His Tag at 0.5 ug/mL (100 uL/well), dose response curve for Human SIRP alpha, Fc Tag with the EC₅₀ of 96.15 ng/mL determined by ELISA.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

CD47 (Human) Recombinant Protein (Cat #P6756) was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-60 kDa.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from PBS, pH 7.4.

Storage Instruction

Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.

Note

Result of activity analysis