CD47 (Human) Recombinant Protein
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Specification
Product Description
Human CD47 (Q08722, 19 a.a. - 139 a.a.) partial recombinant protein with His tag at C-terminus expressed in mammals.
Host
Human
Theoretical MW (kDa)
45-60
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293) expression system
Purification
Ni-sepharose purification
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
Immobilized Human CD47, His Tag at 0.5 ug/mL (100 uL/well), dose response curve for Human SIRP alpha, Fc Tag with the EC50 of 96.15 ng/mL determined by ELISA.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
CD47 (Human) Recombinant Protein (Cat #P6756) was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-60 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — CD47
Entrez GeneID
961Protein Accession#
Q08722Gene Name
CD47
Gene Alias
IAP, MER6, OA3
Gene Description
CD47 molecule
Omim ID
601028Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD47 antigen|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associa
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Interactome
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Pathway
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