CD33 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CD33 (NP_001763.3, 18 a.a. - 259 a.a.) partial recombinant protein with a Fc, 6xHis tag at the C-terminus expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 97% (determined by SDS-PAGE)
Endotoxin Level
< 0.1 EU/ug of protein (determined by LAL method)
Activity
Measured by the binding ability in a functional ELISA. Immobilized human CD33 at 2 ug/mL (100 uL/well) can bind Anti-Human CD33 Antibody with a linear range of 8-20 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Storage Instruction
Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — CD33
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Interactome
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Pathway
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Disease
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