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Last updated: 2022/5/22

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CD86 (Human) Recombinant Protein BioActive

  • Catalog # : P6747
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  • Specification
  • Product Description:
  • Human CD86 (NP_008820.2, 26 a.a. - 247 a.a.) partial recombinant protein with a 6×His tag at the C-terminus expressed in HEK293 cells
  • Host:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purification:
  • Ni-sepharose purification
  • Purity:
  • > 95% (determined by SDS-PAGE)
  • Endotoxin Level:
  • < 0.1 EU/ug of protein (determined by LAL method)
  • Activity:
  • Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD86 at 2 ug/mL (100 uL/well) can bind recombinant human CTLA4 with a linear range of 10-40 ng/mL.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6747
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
  • Storage Instruction:
  • Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years.
    After reconstitution with deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 942
  • Gene Name:
  • CD86
  • Gene Alias:
  • B7-2,B70,CD28LG2,LAB72,MGC34413
  • Gene Description:
  • CD86 molecule
  • Gene Summary:
  • This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
  • Other Designations:
  • B-lymphocyte activation antigen B7-2,B7-2 antigen,CD28 antigen ligand 2,CD86 antigen,CD86 antigen (CD28 antigen ligand 2, B7-2 antigen),CTLA-4 counter-receptor B7.2
  • Interactome
  • Interactome
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