CD80 (Human) Recombinant Protein
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Specification
Product Description
Human CD80 (NP_005182.1, 35 a.a. - 242 a.a.) partial recombinant protein with a 6xHis tag at the C-terminus expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 92% (determined by SDS-PAGE)
Endotoxin Level
< 0.1 EU/ug of protein (determined by LAL method)
Activity
Measured by the binding ability in a functional ELISA. Immobilized recombinant human CD80 at 2 ug/mL (100 uL/well) can bind recombinant human CTLA4 with a linear range of 10-50 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Storage Instruction
Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — CD80
Entrez GeneID
941Protein Accession#
P33681Gene Name
CD80
Gene Alias
CD28LG, CD28LG1, LAB7
Gene Description
CD80 molecule
Omim ID
112203Gene Ontology
HyperlinkGene Summary
The B-lymphocyte activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.[supplied by OMIM
Other Designations
B-lymphocyte activation antigen B7|CD80 antigen|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|costimulatory factor CD80|costimulatory molecule variant IgV-CD80
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Interactome
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Disease
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