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Last updated: 2022/5/15

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CD28 (Human) Recombinant Protein BioActive

  • Catalog # : P6745
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  • Specification
  • Product Description:
  • Human CD28 (NP_006130.1, 19 a.a. - 152 a.a.) partial recombinant protein with a 6×His tag at the C-terminus expressed in HEK293 cells
  • Host:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purification:
  • Ni-sepharose purification
  • Purity:
  • > 97% (determined by SDS-PAGE)
  • Endotoxin Level:
  • < 0.1 EU/ug of protein (determined by LAL method)
  • Activity:
  • Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD28 at 10 ug/mL (100 uL/well) can bind biotinylated human CD86 with a linear range of 3-12 ug/mL.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6745
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
  • Storage Instruction:
  • Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years.
    After reconstitution with deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 940
  • Gene Name:
  • CD28
  • Gene Alias:
  • MGC138290,Tp44
  • Gene Description:
  • CD28 molecule
  • Gene Summary:
  • CD28 costimulation is essential for CD4 (MIM 186940)-positive T-cell proliferation, survival, interleukin-2 (IL2; MIM 147680) production, and T-helper type-2 (Th2) development.[supplied by OMIM
  • Other Designations:
  • CD28 antigen,CD28 antigen (Tp44),T-cell-specific surface glycoprotein
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