Human CD4 (26 a.a. - 390 a.a.) partial recombinant protein with His and Avi tag at the C-terminus expressed in Expi293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (Expi293) expression system
Purification:
Ni-sepharose purification
Purity:
> 95% (determined by Tris-Bis PAGE)
Endotoxin Level:
< 1 EU/ug of protein (determined by LAL method)
Activity:
Immobilized human CD4 at 0.5 ug (100 uL/well) on the plate. Dose response curve for Anti-CD4 Ab with the EC50 of 9.8 ng/mL determined by ELISA.
Quality Control Testing:
Tris-Bis PAGE under reduced condition.
Recommend Usage:
Tris-Bis PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Storage Instruction:
Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.