CD4 (Human) Recombinant Protein
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Specification
Product Description
Human CD4 (26 a.a. - 390 a.a.) partial recombinant protein with His and Avi tag at the C-terminus expressed in Expi293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293) expression system
Purification
Ni-sepharose purification
Purity
> 95% (determined by Tris-Bis PAGE)
Endotoxin Level
< 1 EU/ug of protein (determined by LAL method)
Activity
Immobilized human CD4 at 0.5 ug (100 uL/well) on the plate. Dose response curve for Anti-CD4 Ab with the EC50 of 9.8 ng/mL determined by ELISA.
Quality Control Testing
Tris-Bis PAGE under reduced condition.
Recommend Usage
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Storage Instruction
Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — CD4
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Interactome
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Pathway
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Disease
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