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CD3E/CD3G (Human) Recombinant Protein BioActive

  • Catalog # : P6741
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  • Specification
  • Product Description:
  • Human CD3E/CD3G (P07766, 23 a.a. - 126 a.a./P09693, 23 a.a. - 116 a.a.) partial recombinant protein with a Fc tag at the C-terminus expressed in mammalian cells.
  • Host:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (Expi293) expression system
  • Purification:
  • Protein A purification
  • Purity:
  • > 95% (determined by SDS-PAGE)
  • Endotoxin Level:
  • < 0.1 EU/ug of protein (determined by LAL method)
  • Activity:
  • Immobilized human CD3E/CD3G heterodimer protein at 0.5 ug/mL (100 uL/well), dose response curve for Anti-hCD3e mAb with the EC50 of 1.22 ug/mL determined by ELISA.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6741
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
  • Storage Instruction:
  • Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years.
    After reconstitution with deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Conformation:
  • Heterodimer
  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 916
  • Protein Accession#:
  • P07766;P09693
  • Gene Name:
  • CD3E
  • Gene Alias:
  • FLJ18683,T3E,TCRE
  • Gene Description:
  • CD3e molecule, epsilon (CD3-TCR complex)
  • Gene Summary:
  • The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq
  • Other Designations:
  • CD3-epsilon,CD3E antigen, epsilon polypeptide,CD3e antigen, epsilon polypeptide (TiT3 complex),T-cell antigen receptor complex, epsilon subunit of T3,T-cell surface antigen T3/Leu-4 epsilon chain,T-cell surface glycoprotein CD3 epsilon chain
  • Gene Information
  • Entrez GeneID:
  • 917
  • Protein Accession#:
  • P07766;P09693
  • Gene Name:
  • CD3G
  • Gene Alias:
  • CD3-GAMMA,FLJ17620,FLJ17664,FLJ79544,FLJ94613,MGC138597,T3G
  • Gene Description:
  • CD3g molecule, gamma (CD3-TCR complex)
  • Gene Summary:
  • The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq
  • Other Designations:
  • CD3G antigen, gamma polypeptide,CD3g antigen, gamma polypeptide (TiT3 complex),T-cell antigen receptor complex, gamma subunit of T3,T-cell receptor T3 gamma chain,T-cell surface glycoprotein CD3 gamma chain
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