Human CD3E (P07766, 23 a.a. - 126 a.a.) C119S, C222S mutant partial recombinant protein with a hFc tag at the C-terminus expressed in mammalian cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell expression system
Purification:
Protein A purification
Purity:
> 95% (determined by SDS-PAGE)
Endotoxin Level:
< 1 EU/ug of protein (determined by LAL method)
Activity:
Immobilized human CD3E at 1 ug/mL (100 uL/well). Dose response curve for Anti-CD3 Ab. with the EC50 of 0.1 ug/mL determined by ELISA.
Quality Control Testing:
Tris-Bis PAGE under reduced condition.
Recommend Usage:
Tris-Bis PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4 (5% Trehalose).
Storage Instruction:
Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq