Products

Product Description

Human PDCD1 (Q8IX89, 25 a.a. - 167 a.a.) partial recombinant protein with 6xHis tag at C-terminal expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Host

Human

Form

Lyophlized

Preparation Method

Mammalian cell (HEK293) expression system

Purification

Ni-sepharose purification

Purity

> 95% by SDS-PAGE.

Endotoxin Level

< 0.1 EU/ug of the protein by LAL method.

Activity

Immobilized PDCD1 (Human) Recombinant Protein (Cat # P6733) at 0.5 ug/mL (100 uL/well), dose response curve for Anti-PD1 Ab (BMS) with the ECsub>50 of 57 ng/mL determined by ELISA.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-55 kDa.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from 0.22 um filtered solution of PBS, pH 7.4.

Storage Instruction

Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution with a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis