PVRL1 (Human) Recombinant Protein
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Specification
Product Description
Human PVRL1 (NP_002846.3, 1 a.a. - 334 a.a.) partial recombinant protein with 6xHis tag at C-terminal expressed in HEK293 cells.
Host
Human
Form
Lyophlized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 97% by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ug of the protein by LAL method.
Activity
Measured by the binding ability in a functional ELISA. Immobilized PVRL1 (Human) Recombinant Protein (Cat # P6732) 3 ug/mL (100 uL/well) can bind Recombinant Human Nectin-3 with a linear range of 180-720 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 50-55 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution of PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution with a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — PVRL1
Entrez GeneID
5818Protein Accession#
NP_002846.3; Q15223-1Gene Name
PVRL1
Gene Alias
CD111, CLPED1, ED4, HIgR, HVEC, MGC142031, MGC16207, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12, nectin-1
Gene Description
poliovirus receptor-related 1 (herpesvirus entry mediator C)
Gene Ontology
HyperlinkOther Designations
ectodermal dysplasia 4 (Margarita Island type)|herpesvirus entry mediator C|nectin 1|poliovirus receptor related|poliovirus receptor-like 1|poliovirus receptor-related 1|poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin)
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Interactome
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Disease
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