ACE2 (K353G) (Human) Recombinant Protein
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More Files
- More Functions
Western Blot (Recombinant protein)
Western Blot analysis of Lane 1: 293F negative control, Lane 2: 293F transfection IgG2-ACE2-353G-hFC supernatant of cell cracking fluid and Lane 3: 293F transfection IgG2-ACE2-353G-hFC cell supernatant.
Primary antibody: ACE2 polyclonal antibody (Cat # PAB31772) (1:1000)
Second antibody: Goat anti-Rabbit IgG (H+L)-HPR(1:20000)
Exposure time: 3 s
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Specification
Product Description
Human ACE2 (Q9BYF1-1, 18 a.a. - 740 a.a.) K353G mutant partial recombinant protein with C-terminus hFc tag expressed in 293F cells.
Host
Human
Theoretical MW (kDa)
111.7, 125-150 (R)
Form
Liquid
Preparation Method
Mammalian cell (293F) expression system
Purification
Ni-NTA affinity purification
Purity
> 95% by SDS-PAGE.
Quality Control Testing
2 ug loaded SDS-PAGE
Recommend Usage
ELISA
SDS-PAGE
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
The protein has a calculated MW of 111.7 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of Lane 1: 293F negative control, Lane 2: 293F transfection IgG2-ACE2-353G-hFC supernatant of cell cracking fluid and Lane 3: 293F transfection IgG2-ACE2-353G-hFC cell supernatant.
Primary antibody: ACE2 polyclonal antibody (Cat # PAB31772) (1:1000)
Second antibody: Goat anti-Rabbit IgG (H+L)-HPR(1:20000)
Exposure time: 3 sEnzyme-linked Immunoabsorbent Assay
SDS-PAGE
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Gene Info — ACE2
Entrez GeneID
59272Protein Accession#
Q9BYF1-1Gene Name
ACE2
Gene Alias
ACEH, DKFZp434A014
Gene Description
angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Omim ID
300335Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq
Other Designations
ACE-related carboxypeptidase|OTTHUMP00000022963|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein
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Interactome
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Pathway
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Disease
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