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Last updated: 2022/8/14

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ACE2 (W349G) (Human) Recombinant Protein 

  • Catalog # : P6719
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  • Specification
  • Product Description:
  • Human ACE2 (Q9BYF1-1, 18 a.a. - 740 a.a.) W349G mutant partial recombinant protein with C-terminus hFc tag expressed in 293F cells.
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 111.7, 125-150 (R)
  • Form:
  • Liquid
  • Preparation Method:
  • Mammalian cell (293F) expression system
  • Purification:
  • Ni-NTA affinity purification
  • Purity:
  • > 95% by SDS-PAGE.
  • Quality Control Testing:
  • 2 ug loaded SDS-PAGE

    QC Testing of P6719
  • Recommend Usage:
  • ELISA
    SDS-PAGE
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4.
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • The protein has a calculated MW of 111.7 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot analysis of Lane 1: 293F negative control, Lane 2: 293F transfection IgG2-ACE2-349G-hFC supernatant of cell cracking fluid and Lane 3: 293F transfection IgG2-ACE2-349G-hFC cell supernatant.
    Primary antibody: ACE2 polyclonal antibody (Cat # PAB31772) (1:1000)
    Second antibody: Goat anti-Rabbit IgG (H+L)-HPR(1:20000)
    Exposure time: 30 s
  • Enzyme-linked Immunoabsorbent Assay
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • ACE2
  • Gene Alias:
  • ACEH,DKFZp434A014
  • Gene Description:
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • Gene Summary:
  • The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq
  • Other Designations:
  • ACE-related carboxypeptidase,OTTHUMP00000022963,angiotensin I converting enzyme 2,angiotensin converting enzyme-like protein
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