S (N501Y) (SARS-CoV-2 Alpha Variant) RBD Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Result data
Result data
A. Immobilized S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for human ACE2, Fc tag with the EC50 of 12.7 ng/mL determined by ELISA.
B. The purity of S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein is greater than 95% as determiend by SEC-HPLC.
C. Human ACE2 captured in protein A chip, can bind S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein with an affinity constant of 1.74 nM as determined in SPR assap (Biacore T200).
-
Specification
Product Description
SARS-CoV-2 Alpha Variant S RBD (QHD43416.1, 319 a.a. - 541 a.a.) N501Y mutant recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC.Endotoxin Level
Endotoxin level is less than 1 EU per ug by the LAL method.
Activity
Immobilized S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for human ACE2, Fc tag with the EC50 of 12.7 ng/mL determined by ELISA.
Quality Control Testing
Tris-Bis PAGE under reduced condition
Recommend Usage
ELISA
SDS-PAGE
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM PB, pH 7.4 (5% Trehalose).
Storage Instruction
Store in working aliquots at -20°C.
After reconstitution, store at -20°C for several months or store at -80°C for a year.
Aliquot to avoid repeated freezing and thawing.Note
Result data
-
Applications
Western Blot (Recombinant protein)
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com