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Last updated: 2022/5/22

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S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein CoronavirusBioActive

  • Catalog # : P6709
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  • Specification
  • Product Description:
  • SARS-CoV-2 Variant B.1.1.7 S RBD (QHD43416.1, 319 a.a. - 541 a.a.) N501Y mutant recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.
  • Host:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purity:
  • > 95% as determined by Bis-Tris PAGE
    > 95% as determined by HPLC.
  • Endotoxin Level:
  • Endotoxin level is less than 1 EU per ug by the LAL method.
  • Activity:
  • Immobilized S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for human ACE2, Fc tag with the EC50 of 12.7 ng/mL determined by ELISA.
  • Quality Control Testing:
  • Tris-Bis PAGE under reduced condition

    QC Testing of P6709
  • Recommend Usage:
  • ELISA
    SDS-PAGE
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM PB, pH 7.4 (5% Trehalose).
  • Storage Instruction:
  • Store in working aliquots at -20°C.
    After reconstitution, store at -20°C for several months or store at -80°C for a year.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result data
  • A. Immobilized S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for human ACE2, Fc tag with the EC50 of 12.7 ng/mL determined by ELISA.
    B. The purity of S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein is greater than 95% as determiend by SEC-HPLC.
    C. Human ACE2 captured in protein A chip, can bind S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein with an affinity constant of 1.74 nM as determined in SPR assap (Biacore T200).


  • Applications
  • Western Blot (Recombinant protein)
  • Enzyme-linked Immunoabsorbent Assay
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Enzyme-linked Immunoabsorbent Assay
  • SDS-PAGE
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