S (SARS-CoV-2 Gamma Variant) RBD Recombinant Protein
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Specification
Product Description
SARS-CoV-2 Gamma Variant S RBD (GISAID No. EPI_ISL_833172, 319 a.a. - 541 a.a.) K417T, E484K, N501Y mutant recombinant protein with 6xHis tag at C-terminus expressed in HEK293 cells.
Sequence
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGTIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Host
Human
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Affinity purification with Nickel Columns
Purity
>= 95% by SDS-PAGE.
Endotoxin Level
< 0.1 EU per 1 ug of the protein by LAL test.
Quality Control Testing
SDS-PAGE
Recommend Usage
ELISA
SDS-PAGE
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS.
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
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