S1 (SARS-CoV-2 Alpha Variant) Recombinant Protein
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Specification
Product Description
SARS-CoV-2 Alpha Variant S1 (QIC53204.1, 16 a.a. - 683 a.a.) amino acids 69-70 and 144 deleted; N501Y/A570D/P681H mutant partial recombinant protein with polyHis tag at C-terminal expressed in HEK cells.
Host
Human
Theoretical MW (kDa)
76
Form
Lyophlized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Affinity chromatography nickle column
Purity
greater than 95%.
Endotoxin Level
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1 EU/ug).
Activity
Immobilized human ACE2, His Tag at 0.5 ug/mL (100 ul/Well). Dose response curve for this protein with the ED50 of 0.1 ug/mL determined by ELISA.
Quality Control Testing
1 ug loaded Tris-Bis PAGE under reducing condition.
The purity is greater than 95%.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffer.
After reconstitution with distilled water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This molecule has a calculated mass of approximately 76 kDa. Recombinant S1 migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE.
Result of activity analysis -
Applications
SDS-PAGE
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