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Last updated: 2021/4/11

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N (SARS-CoV-2) Recombinant Protein 

  • Catalog # : P6687
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  • Specification
  • Product Description:
  • SARS-CoV-2 N (P0DTC9) full-length recombinant protein with His tag at C-terminal expressed in HEK293 cells.
  • Host:
  • Human
  • Form:
  • Liquid
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purification:
  • Ni-NTA column
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • SDS-PAGE analysis under reduced and non-reduced conditions.

    QC Testing of P6687
  • Recommend Usage:
  • ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 1X PBS (0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • N (SARS-CoV-2) Recombinant Protein (Cat # P6687) is used as control antigen for assays using recombinant rabbit monoclonal antibody clones 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), and 85C1 (Cat # RAB01059) including in biotinylated format clones 75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), and 85C1 (Cat # RAB01060). These antibodies can be paired in sandwich detection assay and used to detect NP antigen from SARS-CoV-2 with high sensitivity (to 2.5 pg) and high specificity.
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and SARS-CoV NP at 1 ug/mL. Purified recombinant rabbit monoclonal antibodies 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), and 84D7 (Cat # RAB01057) were serially diluted 1:2 starting at 1 ug/mL. Recombinant rabbit monoclonal antibodies 75G5a, 84C4a, and 84D7 show very strong and specific binding to SARS-CoV-2 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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