N (SARS-CoV-2) Recombinant Protein
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Specification
Product Description
SARS-CoV-2 N (QHD43423.2, 1 a.a.- 419 a.a.) full-length recombinant protein with His-SUMO tag at N-terminal expressed in HEK293 cells.
Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Host
Human
Reactivity
SARS-CoV-2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-NTA chromatography
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
SDS-PAGE analysis of N (SARS-CoV-2) Recombinant Protein.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store at -20°C on dry atmosphere.
Aftern reconstitution with deionized water and concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved, store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
SDS-PAGE
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