N (SARS-CoV-2) Recombinant Protein
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Specification
Product Description
SARS-CoV-2 N (QHD43423.2, 1 a.a. - 419 a.a.) recombinant protein with His tag at N-terminal expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
49.4
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Affinity purification
Purity
> 95% by SDS-PAGE; > 95% by HPLC.
Endotoxin Level
< 1.0 EU/ug of the protein by LAL method.
Activity
Immobilized N (SARS-CoV-2) Recombinant Protein at 2 ug/mL (100 uL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-6 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55 kDa.
Storage Buffer
In 20 mM Tris, 500 mM NaCl, pH 8.0 (0.1 mM EDTA, 10% glycerol).
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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