SARS-CoV-2 S ECD (QHD43416.1, 15 a.a. - 1208 a.a.) R682G/R683S/R685S/K986P/V987P mutant partial recombinant protein with His tag at C-terminal expressed in HEK293 cells.
Host:
Human
Theoretical MW (kDa):
132.9
Form:
Liquid
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Affinity purification
Purity:
> 97% by SDS-PAGE.
Endotoxin Level:
< 1.0 EU/ug of the protein by LAL method.
Activity:
Measured by its binding ability in a functional ELISA. Immobilized S (SARS-CoV-2) ECD Recombinant Protein at 2 ug/mL (100 uL/well) can bind recombinant human ACE2 with a linear range of 1.5-35.1 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 170-220 kDa.
Storage Buffer:
In PBS, pH 7.4.
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.