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Product Description

SARS-CoV-2 S1 (YP_009724390.1, 11 a.a. - 682 a.a.) recombinant protein with Fc-His tag at C-terminal expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,SARS-CoV-2,Coronavirus,COVID-19,COVID19,Wuhan virus,Wuhanvirus,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Host

Human

Theoretical MW (kDa)

103

Form

Liquid

Preparation Method

Mammalian cell (HEK293) expression system

Purification

Affinity purification

Purity

> 95% by SDS-PAGE.

Endotoxin Level

< 1.0 EU/ug of the protein by LAL method.

Activity

A. Measured by the binding ability in a functional ELISA. Immobilized recombinant human ACE2 at 2 ug/mL (100 uL/well) can bind S1 (SARS-CoV-2) Recombinant Protein with a linear range of 78-82.5 ng/mL. B. Immobilized human ACE2 on COOH Chip, can bind this protein with an affinity constant of 90.8 nM as determined in a SPR assay (Nicoya OpenSPR).

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Storage Buffer

In PBS, pH 7.4.

Storage Instruction

Store at -80°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis