SARS-CoV-2 S RBD (YP_009724390.1, 319 a.a. - 541 a.a.) recombinant protein with His tag at C-terminal expressed in HEK293 cells.
Host:
Human
Theoretical MW (kDa):
25.9
Form:
Liquid
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Affinity purification
Purity:
> 95% by SDS-PAGE.
Endotoxin Level:
< 0.1 EU/ug of the protein by LAL method.
Activity:
A. Measured by its binding ability in a functional ELISA. Immobilized S (SARS-CoV-2) RBD Recombinant Protein at 2 ug/mL (100 uL/well) can bind recombinant human ACE2 with a linear range of 1.5-9.4 ng/mL. B. Immobilized human ACE2 on COOH Chip, can bind this protein with an affinity constant of 36.7 nM as determined in a SPR assay (Nicoya OpenSPR).
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.
Storage Buffer:
In PBS, pH 7.4.
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.