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N (SARS-CoV-2) Recombinant Protein Coronavirus

  • Catalog # : P6626
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  • Specification
  • Product Description:
  • SARS-CoV-2 N (QHD43423.2) full-length recombinant protein with His tag at C-terminal expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 47.08
  • Specificity:
  • No non-specific binding to normal human serum was identified so far.
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purification:
  • Ni column
  • Purity:
  • > 95% by SDS-PAGE.
  • Quality Control Testing:
  • DTT-reducing SDS-PAGE Result

    QC Testing of P6626
  • Recommend Usage:
  • Antigen
    ELISA
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris, pH 8.5.
  • Storage Instruction:
  • This protein is not stable at 4°C in liquid. For long term storage store at -20 to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Antigen
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA plate was coated by N protein, 100 uL/well, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hour at 37°C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2 M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.
  • SDS-PAGE
  • Application Image
  • Western Blot
  • Antigen
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • SDS-PAGE
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