PIP5K1A (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human PIP5K1A (NP_003548.1, 1 a.a. - 549 a.a.) partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.83
Activity
The activity was determined by ADP-Glo Assay. The enzyme was incubated with Lipid substrate and Mn/ATP. The phosphorylation was detected by the ADP-Glo Kinase Assay technology (luminescent ADP detection assay). Substrate: PI (4) P and Phosphatidylserine, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — PIP5K1A
Entrez GeneID
8394Protein Accession#
NP_003548.1Gene Name
PIP5K1A
Gene Alias
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Gene Description
phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Omim ID
603275Gene Ontology
HyperlinkGene Summary
type I
Other Designations
OTTHUMP00000014290
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Interactome
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Pathway
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