PIKFYVE (S696N/L932S/Q995L/T998S/S1033A/Q1183K) (Human) Recombinant Protein
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SDS-PAGE
The SDS-PAGE of PIKFYVE (S696N/L932S/Q995L/T998S/S1033A/Q1183K) (Human) Recombinant Protein.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human PIKFYVE (NP_055855.2, 1 a.a. - 2098 a.a.) S696N/L932S/Q995L/T998S/S1033A/Q1183K mutant full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.59
Activity
The activity was determined by ADP-Glo Assay. The enzyme was incubated with Lipid substrate and Mn/ATP. The phosphorylation was detected by the ADP-Glo Kinase Assay technology (luminescent ADP detection assay). Substrate: PI (3) P and Phosphatidylserine, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Recommend Usage
Functional Study
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-HCl Buffer, 150 mM NaCl, pH7.5 (0.01% Brij35, 1 mM DTT, 10% glycerol).
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
The SDS-PAGE of PIKFYVE (S696N/L932S/Q995L/T998S/S1033A/Q1183K) (Human) Recombinant Protein. -
Gene Info — PIP5K3
Entrez GeneID
200576GeneBank Accession#
NM_015040.4Protein Accession#
NP_055855.2Gene Name
PIP5K3
Gene Alias
CFD, FAB1, KIAA0981, MGC40423, PIKFYVE, PIP5K
Gene Description
phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
Gene Ontology
HyperlinkGene Summary
PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization (Shisheva et al., 1999 [PubMed 9858586]).[supplied by OMIM
Other Designations
1-phosphatidylinositol-4-phosphate 5-kinase|FYVE finger-containing phosphoinositide kinase|OTTHUMP00000163872|PtdIns(4)P-5-kinase|phosphatidylinositol-3-phosphate 5-kinase type III|phosphatidylinositol-4-phosphate 5-kinase, type III
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Interactome
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Pathway
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Disease
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