PI4KB (Human) Recombinant Protein
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Specification
Product Description
Human PI4KB (NP_002642.1, 1 a.a. - 828 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.7
Activity
The activity was determined by ADP-Glo Assay. The enzyme was incubated with Lipid substrate and Mn/ATP. The phosphorylation was detected by the ADP-Glo Kinase Assay technology (luminescent ADP detection assay). Substrate: PI and Phosphatidylserine, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — PI4KB
Entrez GeneID
5298Protein Accession#
NP_002642.1Gene Name
PI4KB
Gene Alias
PI4K-BETA, PI4KIIIbeta, PI4Kbeta, PIK4CB, pi4K92
Gene Description
phosphatidylinositol 4-kinase, catalytic, beta
Omim ID
602758Gene Ontology
HyperlinkGene Summary
catalytic
Other Designations
OTTHUMP00000014284|phosphatidylinositol 4-kinase, catalytic, beta polypeptide|type III phosphatidylinositol 4-kinase beta
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