ROCK1 (Human) Recombinant Protein
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Specification
Product Description
Human ROCK1 (NP_005397.1, 1 a.a. - 477 a.a.) partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.9399999999999979
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: LIMKtide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.1% CHAPS, 1 mM DTT, 10% glycerol, pH 7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — ROCK1
Entrez GeneID
6093Protein Accession#
NP_005397.1Gene Name
ROCK1
Gene Alias
MGC131603, MGC43611, P160ROCK, PRO0435
Gene Description
Rho-associated, coiled-coil containing protein kinase 1
Omim ID
601702Gene Ontology
HyperlinkGene Summary
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq
Other Designations
OTTHUMP00000162514|p160-ROCK
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