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Last updated: 2019/12/1

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PRKCQ (Human) Recombinant Protein BioActive

  • Catalog # : P6549
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  • Specification
  • Product Description:
  • Human PRKCQ (NP_006248.1, 1 a.a. - 706 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
  • Host:
  • Viruses
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus expression system.
  • Purification:
  • Glutathione sepharose chromatography.
  • Purity:
  • 73%
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg (or Mn)/ATP, and Lipid Activator. Substrate: PKC peptide, ATP: 100 uM.
  • Quality Control Testing:
  • The purity was assessed by SDS-PAGE/CBB staining.
  • Storage Buffer:
  • 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
  • Storage Instruction:
  • Stored at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 5588
  • Gene Name:
  • PRKCQ
  • Gene Alias:
  • MGC126514,MGC141919,PRKCT,nPKC-theta
  • Gene Description:
  • protein kinase C, theta
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019053,OTTHUMP00000043364,OTTHUMP00000043365
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