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Last updated: 2019/8/18

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PRKCH (Human) Recombinant Protein BioActive

  • Catalog # : P6548
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  • Specification
  • Product Description:
  • Human PRKCH (NP_006246.2, 1 a.a. - 683 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
  • Host:
  • Viruses
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus expression system.
  • Purification:
  • Glutathione sepharose chromatography.
  • Purity:
  • 0.7
  • Activity:
  • The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate, Mg (or Mn)/ATP, and Lipid Activator. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: PKC peptide, ATP: 100 uM.
  • Quality Control Testing:
  • The purity was assessed by SDS-PAGE/CBB staining.
  • Storage Buffer:
  • 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
  • Storage Instruction:
  • Stored at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 5583
  • Gene Name:
  • PRKCH
  • Gene Alias:
  • MGC26269,MGC5363,PKC-L,PKCL,PRKCL,nPKC-eta
  • Gene Description:
  • protein kinase C, eta
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. [provided by RefSeq
  • Other Designations:
  • protein kinase C eta type
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