PRKACG (Human) Recombinant Protein
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Specification
Product Description
Human PRKACG (NP_002723.2, 1 a.a. - 351 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.7
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: Kemptide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — PRKACG
Entrez GeneID
5568Protein Accession#
NP_002723.2Gene Name
PRKACG
Gene Alias
KAPG, PKACg
Gene Description
protein kinase, cAMP-dependent, catalytic, gamma
Omim ID
176893Gene Ontology
HyperlinkGene Summary
Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. [provided by RefSeq
Other Designations
OTTHUMP00000021422|PKA C-gamma|serine(threonine) protein kinase
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