CDK16 (S153A)/CCNY (Human) Recombinant Protein
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Specification
Product Description
Human CDK16 S153A mutant/CCNY (NP_006192.1/NP_659449.3) partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.91
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: PCTAIRE1 peptide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — PCTK1
Entrez GeneID
5127Protein Accession#
NP_006192.1;NP_659449.3Gene Name
PCTK1
Gene Alias
FLJ16665, PCTAIRE, PCTAIRE1, PCTGAIRE
Gene Description
PCTAIRE protein kinase 1
Omim ID
311550Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000023208|OTTHUMP00000023209|PCTAIRE-motif protein kinase 1|serine/threonine-protein kinase
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Gene Info — CCNY
Entrez GeneID
219771Protein Accession#
NP_006192.1;NP_659449.3Gene Name
CCNY
Gene Alias
C10orf9, CBCP1, CFP1, FLJ95513
Gene Description
cyclin Y
Gene Ontology
HyperlinkGene Summary
Cyclins, such as CCNY, control cell division cycles and regulate cyclin-dependent kinases (e.g., CDC2; MIM 116940) (Li et al., 2009 [PubMed 18060517]).[supplied by OMIM
Other Designations
OTTHUMP00000019452|OTTHUMP00000019453|cyclin fold protein 1|cyclin-box carrying protein 1
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Interactome
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Disease
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