NEK7 (Human) Recombinant Protein
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Specification
Product Description
Human NEK7 (NP_598001.1, 1 a.a. - 302 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.88
Activity
The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: CDK7 peptide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — NEK7
Entrez GeneID
140609Protein Accession#
NP_598001.1Gene Name
NEK7
Gene Alias
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Gene Description
NIMA (never in mitosis gene a)-related kinase 7
Omim ID
606848Gene Ontology
HyperlinkGene Summary
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM
Other Designations
OTTHUMP00000033680
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Disease
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