NEK6 (Human) Recombinant Protein
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Specification
Product Description
Human NEK6 (NP_055212.2, 1 a.a. - 313 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.88
Activity
The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: CDK7 peptide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — NEK6
Entrez GeneID
10783Protein Accession#
NP_055212.2Gene Name
NEK6
Gene Alias
SID6-1512
Gene Description
NIMA (never in mitosis gene a)-related kinase 6
Omim ID
604884Gene Ontology
HyperlinkGene Summary
The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA.[supplied by OMIM
Other Designations
OTTHUMP00000022095|OTTHUMP00000022097|putative serine-threonine protein kinase
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