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Last updated: 2019/12/8

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ITK (Human) Recombinant Protein BioActive

  • Catalog # : P6506
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  • Specification
  • Product Description:
  • Human ITK (NP_005537.3, 2 a.a. - 620 a.a.) partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
  • Host:
  • Viruses
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus expression system.
  • Purification:
  • Glutathione sepharose chromatography.
  • Purity:
  • 85%
  • Activity:
  • The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: Srctide, ATP: 100 uM.
  • Quality Control Testing:
  • The purity was assessed by SDS-PAGE/CBB staining.
  • Storage Buffer:
  • 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
  • Storage Instruction:
  • Stored at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 3702
  • Gene Name:
  • ITK
  • Gene Alias:
  • EMT,LYK,MGC126257,MGC126258,PSCTK2
  • Gene Description:
  • IL2-inducible T-cell kinase
  • Gene Summary:
  • This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq
  • Other Designations:
  • homolog of mouse T-cell itk/tsk,tyrosine-protein kinase ITK/TSK,tyrosine-protein kinase LYK
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