Human ITK (NP_005537.3, 2 a.a. - 620 a.a.) partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host:
Viruses
Form:
Liquid
Preparation Method:
Baculovirus expression system.
Purification:
Glutathione sepharose chromatography.
Purity:
85%
Activity:
The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: Srctide, ATP: 100 uM.
Quality Control Testing:
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer:
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction:
Stored at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq
Other Designations:
homolog of mouse T-cell itk/tsk,tyrosine-protein kinase ITK/TSK,tyrosine-protein kinase LYK