HIPK1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human HIPK1 (NP_689909.2, 158 a.a. - 555 a.a.) partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.83
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: DYRKtide-F, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — HIPK1
Entrez GeneID
204851Protein Accession#
NP_689909.2Gene Name
HIPK1
Gene Alias
KIAA0630, MGC26642, MGC33446, MGC33548, Myak, Nbak2
Gene Description
homeodomain interacting protein kinase 1
Omim ID
608003Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000013763|OTTHUMP00000013764|OTTHUMP00000013767|OTTHUMP00000013768|homeodomain interacting protein kinase 1-like protein|homeodomain-interacting protein kinase 1|nuclear body associated kinase 2b
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