Human CHEK1 (NP_001265.1, 1 a.a. - 476 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host:
Viruses
Form:
Liquid
Preparation Method:
Baculovirus expression system.
Purification:
Glutathione sepharose chromatography.
Purity:
58%
Activity:
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: CHKtide, ATP: 100 uM.
Quality Control Testing:
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer:
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction:
Stored at -80°C. Aliquot to avoid repeated freezing and thawing.