CHEK1 (Human) Recombinant Protein
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Specification
Product Description
Human CHEK1 (NP_001265.1, 1 a.a. - 476 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.58
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: CHKtide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — CHEK1
Entrez GeneID
1111Protein Accession#
NP_001265.1Gene Name
CHEK1
Gene Alias
CHK1
Gene Description
CHK1 checkpoint homolog (S. pombe)
Omim ID
603078Gene Ontology
HyperlinkGene Summary
S. pombe
Other Designations
CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1
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Interactome
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