Product Browser

Last updated: 2019/8/18

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

BUB1/BUB3 (Human) Recombinant Protein BioActive

  • Catalog # : P6474
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Human BUB1/BUB3 (NP_004327/NP_004716) full length recombinant protein with GST/DYKDDDDK-tag at N-terminal using baculovirus expression system.
  • Host:
  • Viruses
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus expression system.
  • Purification:
  • Glutathione sepharose chromatography.
  • Purity:
  • 0.7
  • Activity:
  • The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: H2A peptide, ATP: 100 uM.
  • Quality Control Testing:
  • The purity was assessed by SDS-PAGE/CBB staining.
  • Storage Buffer:
  • 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
  • Storage Instruction:
  • Stored at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 699
  • Protein Accession#:
  • NP_004327;NP_004716
  • Gene Name:
  • BUB1
  • Gene Alias:
  • BUB1A,BUB1L,hBUB1
  • Gene Description:
  • budding uninhibited by benzimidazoles 1 homolog (yeast)
  • Gene Summary:
  • This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq
  • Other Designations:
  • BUB1 budding uninhibited by benzimidazoles 1 homolog,budding uninhibited by benzimidazoles 1,mitotic spindle checkpoint kinase,putative serine/threonine-protein kinase
  • Gene Information
  • Entrez GeneID:
  • 9184
  • Protein Accession#:
  • NP_004327;NP_004716
  • Gene Name:
  • BUB3
  • Gene Alias:
  • BUB3L,hBUB3
  • Gene Description:
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • Gene Summary:
  • This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • BUB3 budding uninhibited by benzimidazoles 3 homolog,OTTHUMP00000020679,OTTHUMP00000020680,budding uninhibited by benomyl,budding uninhibited by benzimidazoles 3,mitotic checkpoint component
  • RSS
  • YouTube
  • Linkedin
  • Facebook