BUB1/BUB3 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human BUB1/BUB3 (NP_004327/NP_004716) full length recombinant protein with GST/DYKDDDDK-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.7
Activity
The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: H2A peptide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — BUB1
Entrez GeneID
699Protein Accession#
NP_004327;NP_004716Gene Name
BUB1
Gene Alias
BUB1A, BUB1L, hBUB1
Gene Description
budding uninhibited by benzimidazoles 1 homolog (yeast)
Omim ID
602452Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
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Gene Info — BUB3
Entrez GeneID
9184Protein Accession#
NP_004327;NP_004716Gene Name
BUB3
Gene Alias
BUB3L, hBUB3
Gene Description
budding uninhibited by benzimidazoles 3 homolog (yeast)
Omim ID
603719Gene Ontology
HyperlinkGene Summary
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
BUB3 budding uninhibited by benzimidazoles 3 homolog|OTTHUMP00000020679|OTTHUMP00000020680|budding uninhibited by benomyl|budding uninhibited by benzimidazoles 3|mitotic checkpoint component
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Interactome
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Pathway
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Disease
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