Product Browser

Last updated: 2019/12/8

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

PRKAA1/PRKAB2/PRKAG1 (Human) Recombinant Protein BioActive

  • Catalog # : P6467
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Human PRKAA1/PRKAB2/PRKAG1 (NP_006242.4/NP_005390.1/NP_002724.1) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system and activated with His-tag CaMKK1.
  • Host:
  • Viruses
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus expression system.
  • Purification:
  • Glutathione sepharose chromatography.
  • Purity:
  • 98%
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: SAMS peptide, ATP: 100 uM.
  • Quality Control Testing:
  • The purity was assessed by SDS-PAGE/CBB staining.
  • Storage Buffer:
  • 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
  • Storage Instruction:
  • Stored at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 5562
  • Protein Accession#:
  • NP_006242.4;NP_005390.1;NP_002724.1
  • Gene Name:
  • PRKAA1
  • Gene Alias:
  • AMPK,AMPKa1,MGC33776,MGC57364
  • Gene Description:
  • protein kinase, AMP-activated, alpha 1 catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase, catalytic alpha-1 chain,AMP -activate kinase alpha 1 subunit,AMP-activated protein kinase, catalytic, alpha-1,AMPK alpha 1
  • Gene Information
  • Entrez GeneID:
  • 5565
  • Protein Accession#:
  • NP_006242.4;NP_005390.1;NP_002724.1
  • Gene Name:
  • PRKAB2
  • Gene Alias:
  • MGC61468
  • Gene Description:
  • protein kinase, AMP-activated, beta 2 non-catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase, beta-2 subunit,AMP-activated protein kinase beta 2 non-catalytic subunit,AMPK beta 2,AMPK beta-2 chain,OTTHUMP00000015910
  • Gene Information
  • Entrez GeneID:
  • 5571
  • Protein Accession#:
  • NP_006242.4;NP_005390.1;NP_002724.1
  • Gene Name:
  • PRKAG1
  • Gene Alias:
  • AMPKG,MGC8666
  • Gene Description:
  • protein kinase, AMP-activated, gamma 1 non-catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase, gamma-1 subunit,AMP-activated protein kinase, noncatalytic gamma-1 subunit,AMPK gamma-1 chain
  • RSS
  • YouTube
  • Linkedin
  • Facebook