AKT2 (Human) Recombinant Protein
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Specification
Product Description
Human AKT2 (NP_001617.1, 120 a.a. - 481 a.a.) partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.72
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: Crosstide, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — AKT2
Entrez GeneID
208Protein Accession#
NP_001617.1Gene Name
AKT2
Gene Alias
PKBB, PKBBETA, PRKBB, RAC-BETA
Gene Description
v-akt murine thymoma viral oncogene homolog 2
Gene Ontology
HyperlinkGene Summary
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Other Designations
Murine thymoma viral (v-akt) homolog-2|rac protein kinase beta
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