BMP2 (Human/Mouse/Rat) Recombinant Protein
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Specification
Product Description
Human/Mouse/Rat INHBA (P12643/P21274/P49001) recombinant protein expressed in E.Coli.
Sequence
MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
Host
Escherichia coli
Theoretical MW (kDa)
13.0/26.1
Form
Lyophilized
Preparation Method
This product is produced with no animal or human origin raw products. All processing and handling employs animal free equipment and animal free protocols.
Purity
>= 95%
Endotoxin Level
<= 1 EUs/ug (Kinetic LAL)
Activity
ED50 <= 250 ng/mL
Alkaline phosphatase activity induced in ATDC-5 cells
The values provided above are minimum expected values to pass internal requirements.Quality Control Testing
Reducing and Non-Reducing SDS PAGE
1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. Human BMP-2 has predicted MW of 26.0 KDa as a homodimer (with each subunit having a MW of 13.0 kDa).
Conformation
Dimer
Storage Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Storage Instruction
Stored at -20°C prior to reconstitution for 1 year.
After reconstitution with sterile water at 0.1 mg/mL, store at 4°C for 1 month. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C for 3 months.
Aliquot to avoid repeated freezing and thawing.
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.Note
Result of activity analysis
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Applications
Western Blot
Functional Study
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Gene Info — BMP2
Entrez GeneID
650Protein Accession#
P12643/P21274/P49001Gene Name
BMP2
Gene Alias
BMP2A
Gene Description
bone morphogenetic protein 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq
Other Designations
OTTHUMP00000030228
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Gene Info — Bmp2
Entrez GeneID
12156Protein Accession#
P12643/P21274/P49001Gene Name
Bmp2
Gene Alias
AI467020, Bmp2a
Gene Description
bone morphogenetic protein 2
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000016666
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Gene Info — Bmp2
Entrez GeneID
29373Protein Accession#
P12643/P21274/P49001Gene Name
Bmp2
Gene Alias
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Gene Description
bone morphogenetic protein 2
Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Disease
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