FGF9 (Human) Recombinant Protein
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Specification
Product Description
Human FGF9 (P31371) recombinant protein expressed in E.Coli.
Sequence
MPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS
Host
Escherichia coli
Theoretical MW (kDa)
23.4
Form
Lyophilized
Purity
>= 95%
Endotoxin Level
<= 1 EUs/ug (Kinetic LAL) (50% confidence)
Activity
ED50 <= 2 ng/mL
3T3 cell proliferation
The values provided above are minimum expected values to pass internal requirements.Quality Control Testing
Reducing and Non-Reducing SDS PAGE
Conformation
Monomer
Storage Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 25 mM NaCl, 50 mM sodium sulfate, pH 7.5.
Storage Instruction
Stored at -20°C to-80°C for 12 month.
After reconstitution with sterile water at 0.1 mg/mL, store at -20°C to -80°C for 3 months, store at 4°C for 1 month.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot
Functional Study
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Gene Info — FGF9
Entrez GeneID
2254Protein Accession#
P31371Gene Name
FGF9
Gene Alias
GAF, HBFG-9, MGC119914, MGC119915
Gene Description
fibroblast growth factor 9 (glia-activating factor)
Omim ID
600921Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000018804|fibroblast growth factor 9|glia-activating factor
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Interactome
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